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| Reactivity: | H,M & H |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Rad51 rabbit polyclonal antibody |
Gene Name: | DNA repair protein RAD51 homolog 1 |
Synonyms: | RAD51, BRCC5, FANCR, HHsRad51, HsT16930, MRMV2, RAD51A, RECA, RAD51 recombinase |
Immunogen: | Recombinant protein corresponding to Mouse Rad51 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 37 kDa |
Observed MW. | 37 kDa |
Uniprot ID: | Q06609, Q08297 |
Product Usage Information
WB | Human, Mouse | 1: 500-1: 1000 | thymus |
ICC/IF | Human | 1: 100-1: 400 | HeLa |
Background
RAD51 is a eukaryotic gene, which is highly conserved from yeast to humans. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks. In humans, RAD51 is a 339-amino acid protein that plays a major role in homologous recombination of DNA during double strand break repair. In this process, an ATP dependent DNA strand exchange takes place in which a template strand invades base-paired strands of homologous DNA molecules. RAD51 is involved in the search for homology and strand pairing stages of the process. Unlike other proteins involved in DNA metabolism, the RecA/Rad51 family forms a helical nucleoprotein filament on DNA. This protein can interact with the ssDNA-binding protein RPA, BRCA2, PALB2 and RAD52.
Images
 |
Western blot analysis of Rad51 (GB11572) at dilution of 1: 500 |
| ICC analysis of Rad51(GB11572)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage |
Store at -20°C for one year. Avoid repeated freeze/thaw cycles |
| Storage Buffer |
PBS with 0.02%sodium azide, 100 μg/ml BSA and 50% glycerol. |