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| Reactivity: | H,M,R & M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | DNA repair protein RAD51 homolog 1 |
Synonyms: | RAD51, BRCC5, FANCR, HHsRad51, HsT16930, MRMV2, RAD51A, RECA, RAD51 recombinase |
Immunogen: | Recombinant protein corresponding to Mouse Rad51 |
Uniprot ID: | Q08297 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoskeleton |
Predicted MW. / Observed MW. | 37 kDa / 37 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus |
IHC/IF | Mouse, Rat | 1: 500-1: 4000 | brain, testis |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa |
Background
RAD51 is a eukaryotic gene, which is highly conserved from yeast to humans. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks. In humans, RAD51 is a 339-amino acid protein that plays a major role in homologous recombination of DNA during double strand break repair. In this process, an ATP dependent DNA strand exchange takes place in which a template strand invades base-paired strands of homologous DNA molecules. RAD51 is involved in the search for homology and strand pairing stages of the process.
Images
 | Western blot analysis of Rad51 (GB11573) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded mouse brain using RAD51 (GB11573) at dilution of 1: 2000 (400x lens) |
 | Immunohistochemistry of paraffin embedded mouse brain using RAD51 (GB11573) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse ovary using RAD51 (GB11573) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse testis using RAD51 (GB11573) at dilution of 1: 2000 (300x lens) |
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Immunohistochemistry of paraffin embedded mouse testis using RAD51 (GB11573) at dilution of 1: 3000 (300x lens) |
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Immunohistochemistry of paraffin embedded rat brain using RAD51 (GB11573) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat ovary using RAD51 (GB11573) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat testis using RAD51 (GB11573) at dilution of 1: 3000 (300x lens) |
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Immunofluorescence of paraffin embedded mouse brain using RAD51 (GB11573) at dilution of 1: 2000 (400x lens) |
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Immunofluorescence of paraffin embedded rat parencephalon using RAD51 (GB11573) at dilution of 1: 2000 (400x lens) |
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Immunofluorescence of paraffin embedded rat prefrontal lobe using RAD51 (GB11573) at dilution of 1: 2000 (400x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using Rad51(GB11573) at dilution of 1:100-1:400 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using Rad51(GB11573) at dilution of 1:100-1:400 |
Storage
| Storage |
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer |
PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol |