| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SERCA2 ATPase rabbit polyclonal antibody |
Gene Name: | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 |
Synonyms: | SERCA2, SR Ca(2+)-ATPase 2, alcium pump 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Atp2a2 |
Immunogen: | Recombinant protein corresponding to Mouse SERCA2 ATPase |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum |
Predicted MW. | 115 kDa |
Observed MW. | 115 kDa |
Uniprot ID: | O55143, P11507 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | heart |
IHC/IF | Mouse, Rat | 1: 250-1: 1000 | heart, skeletal muscle |
Background
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle.
Images
| Western blot analysis of ATP2A2 (GB112371) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded mouse heart using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse skeletal muscle using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) |
| Immunohistochemistry of paraffin embedded rat heart using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) |
| Immunohistochemistry of paraffin embedded rat skeletal muscle using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |