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Anti-SUCLG2 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SUCLG2 rabbit polyclonal |
Gene Name: | Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial |
Synonyms: | GTP-specific succinyl-CoA synthetase subunit beta, G-SCS, GTPSCS, Succinyl-CoA synthetase beta-G chain, SCS-betaG, Suclg2 |
Immunogen: | Recombinant protein corresponding to Mouse SUCLG2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 47 kDa |
Observed MW. | 47 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 2000-1: 4000 | kidney, liver, stomach |
IHC | Human, Mouse, Rat | 1: 1000-1: 2000 | tonsil, colon, para-cancer stomach |
IF | Human, Mouse, Rat | 1: 600-1: 1200 | tonsil, testis, spleen |
Background
GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Images
| Western blot analysis of SUCLG2 (GB112775) at dilution of 1: 4000 Lane 1: Raji cell lysate Lane 2: MCF7 cell lysate Lane 3: Mouse kidney tissue lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse stomach tissue lysate Lane 6: Rat kidney tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human tonsil using SUCLG2 (GB112775) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human colon using SUCLG2 (GB112775) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human para-cancer stomach using SUCLG2 (GB112775) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded rat kidney using SUCLG2 (GB112775) at dilution of 1: 2000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human tonsil using SUCLG2 (GB112775) at dilution of 1: 1200 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using SUCLG2 (GB112775) at dilution of 1: 1200 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat spleen using SUCLG2 (GB112775) at dilution of 1: 1200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |