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Anti-TCF3 / E2A Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Transcription factor E2-alpha |
Synonyms: | bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse TCF3 |
Uniprot ID: | P15806 |
Isotype: | lgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 68 kDa / 70 kDa |
Product Usage Information
WB | Human | 1: 300-1: 400 | HepG2, HCT116, Raji |
Background
Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (By similarity). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
Images
| WB analysis of TCF3 (GB115489). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |