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Anti-Thyrotroph embryonic factor Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Thyrotroph embryonic factor rabbit polyclonal antibody |
Gene Name: | Thyrotroph embryonic factor |
Synonyms: | Tef, TEF Transcription Factor, KIAA1655, PAR BZIP Transcription Factor, PAR BZIP Family Member |
Immunogen: | Recombinantproteincorrespondingto Mouse Thyrotroph embryonic factor |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 33 kDa |
Observed MW. | 40 kDa |
Uniprot ID: | Q9JLC6, P41224 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | brain, cerebral cortex, skeletal muscle |
IHC/IF | Mouse, Rat | 1: 400-1: 1400 | brain, heart, Skeletal muscle |
Background
TEF (thyrotrophic embryonic factor), also known as KIAA1655, is a 303 amino acid nuclear transcription factor that belongs to the bZIP (basic region/leucine zipper) family and PAR (proline and acidic amino acid-rich) subfamily. TEF binds DNA as either a homodimer or heterodimer, and is known to transactivate the TSH beta promoter. While broadly expressed in adults, TEF is only found in developing embryonic anterior pituitary gland.
Images
| Western blot analysis of TEF (GB111653) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded mouse brain using TEF (GB111653) at dilution of 1: 700 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse skeletal muscle using TEF (GB111653) at dilution of 1: 700 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using TEF (GB111653) at dilution of 1: 700 (200x lens) |
| Immunofluorescence of paraffin embedded mouse skeletal muscle using TEF (GB111653) at dilution of 1: 700 (300x lens) |
| Immunofluorescence of paraffin embedded rat brain using TEF (GB111653) at dilution of 1: 700 (200x lens) |
 | Immunofluorescence of paraffin embedded rat heart using TEF (GB111653) at dilution of 1: 700 (300x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |