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Anti-alpha 1 Fetoprotein Mouse mAb (100 μl)
| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Alpha-fetoprotein |
Synonyms: | AFP, AFPD, FETA, HPalpha fetoprotein |
Immunogen: | Recombinant protein corresponding to Human AFP |
Uniprot ID: | P02771 |
Isotype: | IgG2a,κ |
Purity: | Affinity purification |
Subcellular location: | Secreted, Cytoplasm |
Predicted MW. / Observed MW. | 68 kDa / 68-72 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | HeLa, MCF7 |
IHC/IF | Human | 1: 250-1: 1000 | liver, liver cancer |
Background
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult. So AFP is usually considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma.
Images
| Western blot analysis of AFP (GB12288) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human liver using AFP (GB12288) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human liver cancer using AFP (GB12288) at dilution of 1: 250 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |