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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IP, ELISA, RIP |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | TAR DNA binding protein |
| Gene Symbol: | TARDBP |
| Synonyms: | ALS10; TDP-43; TDP-43/TARDBP |
| Gene ID: | 23435 |
| UniProt ID: | Q13148 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TDP-43/TARDBP (NP_031401.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; RIP,1:20-1:50 |
| Purification Method: | Affinity purification |
| Concentration: | 1.63 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-TARDBP antibody |
Background
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Images
 | Western blot analysis of various lysates using TDP-43/TARDB Rabbit pAb (A1183) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry analysis of paraffin-embedded Rat spleen using TDP-43/TARDB Rabbit pAb (A1183) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using TDP-43/TARDB Rabbit pAb (A1183) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain using TDP-43/TARDB Rabbit pAb (A1183) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunoprecipitation analysis of 200 μg extracts of SW620 cells using 3 μg TDP-43/TARDB antibody (A1183). Western blot was performed from the immunoprecipitate using TDP-43/TARDB antibody (A1183) at a dilution of 1:500. |
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