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| Reactivity: | R & M,R & M,R |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | TBXT rabbit polyclonal |
Gene Name: | T-box transcription factor T |
Synonyms: | Brachyury protein, Protein T, Tbxt, T |
Immunogen: | Recombinant protein corresponding to Mouse TBXT |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 47 kDa |
Observed MW. | 47 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Rat | 1: 700-1: 1000 | testis |
IHC | Mouse, Rat | 1: 1600-1: 3200 | testis, substantia nigra, striatum, hippocampus |
IF | Mouse, Rat | 1: 300-1: 600 | substantia nigra |
Background
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation . Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
Images
| Western blot analysis of TBXT (GB112719) at dilution of 1: 1000 Lane 1: Mouse testis tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using TBXT (GB112719) at dilution of 1: 3200 |
| Immunohistochemistry analysis of paraffin-embedded rat testis using TBXT (GB112719) at dilution of 1: 3200 |
| Immunohistochemistry analysis of paraffin-embedded mouse substantia nigra using TBXT (GB112719) at dilution of 1: 3200 |
| Immunohistochemistry analysis of paraffin-embedded rat substantia nigra using TBXT (GB112719) at dilution of 1: 3200 |
| Immunohistochemistry analysis of paraffin-embedded mouse striatum using TBXT (GB112719) at dilution of 1: 3200 |
| Immunohistochemistry analysis of paraffin-embedded rat hippocampus using TBXT (GB112719) at dilution of 1: 3200 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse substantia nigra using TBXT (GB112719) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat substantia nigra using TBXT (GB112719) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |