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Cardiac Troponin I (TNNI3) Rabbit pAb (20 μl)
| Reactivity: | Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | troponin I3, cardiac type |
| Gene Symbol: | TNNI3 |
| Synonyms: | CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; Cardiac Troponin I (TNNI3) |
| Gene ID: | 7137 |
| UniProt ID: | P19429 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human Cardiac Troponin I (TNNI3) (NP_000354.4). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.86 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TNNI3 antibody |
Background
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19.
Images
 | Western blot analysis of lysates from Mouse heart, using Cardiac Troponin I (TNNI3) Rabbit pAb (A0152) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Western blot analysis of lysates from Rat lung, using Cardiac Troponin I (TNNI3) Rabbit pAb (A0152) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of paraffin-embedded mouse heart using Cardiac Troponin I (TNNI3) Rabbit pAb (A0152) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 600 μg extracts of Mouse heart cells using 3 μg Cardiac Troponin I (TNNI3) antibody (A0152). Western blot was performed from the immunoprecipitate using Cardiac Troponin I (TNNI3) antibody (A0152) at a dilution of 1:1000. |
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