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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | tRNA methyltransferase 2 homolog A |
| Gene Symbol: | TRMT2A |
| Synonyms: | HTF9C; TRMT2A/HTF9C |
| Gene ID: | 27037 |
| UniProt ID: | Q8IZ69 |
| Clone ID: | 2U3W8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRMT2A/HTF9C (Q8IZ69). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.50 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TRMT2A antibody |
Background
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from K-562 cells, using TRMT2A/HTF9C Rabbit mAb (A0136) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from MCF7 cells, using TRMT2A/HTF9C Rabbit mAb (A0136) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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