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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | thiosulfate sulfurtransferase |
| Gene Symbol: | TST |
| Synonyms: | RDS; TST |
| Gene ID: | 7263 |
| UniProt ID: | Q16762 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2). |
| Dilution: | WB 1:1000-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.98 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TST antibody |
Background
This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using TST Rabbit pAb (A10542) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver using TST Rabbit pAb (A10542) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using TST Rabbit pAb (A10542) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunoprecipitation analysis of 200 μg extracts of A-549 cells, using 3 μg TST antibody (A10542). Western blot was performed from the immunoprecipitate using TST antibody (A10542) at a dilution of 1:1000. |
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