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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | ubiquitin protein ligase E3 component n-recognin 7 |
| Gene Symbol: | UBR7 |
| Synonyms: | LICAS; C14orf130; UBR7 |
| Gene ID: | 55148 |
| UniProt ID: | Q8N806 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 76-425 of human UBR7 (NP_786924.2). |
| Dilution: | WB 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 4.24 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-UBR7 antibody |
Background
This gene encodes a UBR box-containing protein that belongs to the E3 ubiquitin ligase family. The protein also contains a plant homeodomain (PHD) in the C-terminus. In mammals, the encoded protein recognizes N-degrons, the destabilizing N-terminal residues of short-lived proteins, which results in ubiquitinylation, and proteolysis via the proteasome.
Images
 | Western blot analysis of various lysates using UBR7 Rabbit pAb (A15464) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Western blot analysis of various lysates using UBR7 Rabbit pAb (A15464) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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