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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | ubiquinol-cytochrome c reductase, complex III subunit X |
| Gene Symbol: | UQCR10 |
| Synonyms: | QCR9; UCRC; HSPC051; HSPC119; HSPC151; UCCR7.2; UQCR10 |
| Gene ID: | 29796 |
| UniProt ID: | Q9UDW1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human UQCR10 (NP_037519.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.38 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-UQCR10 antibody |
Background
UCRC is a subunit of mitochondrial complex III (ubiquinol-cytochrome c reductase; EC 1.10.2.2), which forms the middle segment of the respiratory chain of the inner mitochondrial membrane (Schagger et al., 1995 [PubMed 8592474]).
Images
 | Western blot analysis of various lysates using UQCR10 Rabbit pAb (A12587) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat ovary using UQCR10 Rabbit pAb (A12587) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat heart using UQCR10 Rabbit pAb (A12587) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Confocal immunofluorescence analysis of U2OS cells using UQCR10 Rabbit pAb (A12587) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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