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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | X-ray repair cross complementing 1 |
| Gene Symbol: | XRCC1 |
| Synonyms: | RCC; SCAR26; XRCC1 |
| Gene ID: | 7515 |
| UniProt ID: | P18887 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-633 of human XRCC1 (NP_006288.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:100-1:500; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.34 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-XRCC1 antibody |
Background
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Images
 | Western blot analysis of various lysates using XRCC1 Rabbit pAb (A0442) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using XRCC1 Rabbit pAb (A0442) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse lung using XRCC1 Rabbit pAb (A0442) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of HeLa cells using XRCC1 Rabbit pAb (A0442) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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