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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta |
| Gene Symbol: | YWHAB |
| Synonyms: | HS1; GW128; YWHAA; KCIP-1; HEL-S-1; 14-3-3 alpha/beta |
| Gene ID: | 7529 |
| UniProt ID: | P31946 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-246 of human 14-3-3 alpha/beta (NP_003395.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.53 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-YWHAB antibody |
Background
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
Images
 | Western blot analysis of various lysates using 14-3-3 alpha/beta Rabbit pAb (A1023) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil using 14-3-3 alpha/beta Rabbit pAb (A1023) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human appendix using 14-3-3 alpha/beta Rabbit pAb (A1023) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of HeLa cells using 14-3-3 alpha/beta Rabbit pAb (A1023) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using 14-3-3 alpha/beta Rabbit pAb (A1023) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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