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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA, ChIP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | YY1 transcription factor |
| Gene Symbol: | YY1 |
| Synonyms: | DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1; YY1 |
| Gene ID: | 7528 |
| UniProt ID: | P25490 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-160 of human YY1 (NP_003394.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.52 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-YY1 antibody |
Background
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Images
 | Western blot analysis of various lysates using YY1 Rabbit pAb (A12928) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (A12928) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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