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Anti-p19 INK4d Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cyclin-dependent kinase 4 inhibitor D |
Synonyms: | CDKN2D, INK4D, p19, p19 INK4d, cyclin dependent kinase inhibitor 2D, CDK inhibitor p19INK4d, Nur77 associating protein |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse p19 INK4d |
Uniprot ID: | Q60773 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 19 kDa / 19 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 250-1: 500 | HeLa, HepG2, A431 |
ICC/IF | Human | 1: 100-1: 300 | HeLa |
Background
Cyclin-dependent kinase 4 inhibitor D is an enzyme that in humans is encoded by the CDKN2D gene.The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase.
Images
| WB analysis of p19 INK4d(GB11419). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 250, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
|
ICC analysis of p19 INK4d(GB11419)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |