5-Ethynyl-dUTP (5-EdUTP) is supplied as a 1 mM solution in TE buffer. 5-EdUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.
Specifications
1. Concentration: 1 mM solution in TE buffer
2. Molecular Formula: C11H15N2O14P3 (free acid)
3. Molecular Weight: 492.16 (free acid)
Applications
Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension.
Component
1. 5-Ethynyl-dUTP (5-EdUTP): 500 μl
Storage
Store at -20°C
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