Transfection Reagents

Transfection Reagents

Based on our unique polymer synthesis technologies, currently we are developing and manufacturing four categories of transfection reagents, which show distinct transfection characteristics compared with the leading products in the market. What's more important is that the transfection reagents we are manufacturing are very affordable and will save your precious research budget.

Order Guide

Sample & Cell Type
PeneFect™ > Excellent for both single DNA transfection and 
   multi DNA co-transfection

> Simple & robust transfection procedure
> Cost-effective

Mammalian cells

Lipofectamine 2000

LiFect293™ > Best for suspension 293 cells 
> High levels of recombinant protein production
> Presence of serum and antibiotics enhances efficiency on 293 cells
> Exceptional efficiency for both single DNA transfection and multi DNAs co-transfection
HEK293 related cells
293Fectin, Fugene 6
LiFectRNA™ > Excellent silencing at low concentrations with only 1.0 nM siRNA / miRNA mimics  
> inimizing non-specific and off target effects in the cell
> Best for broad mammalian cells
> Very low cytotoxicity
Mammalian cells
293Fectin, Fugene 6
PrimeFect™ > Superior transfection efficiency for a broad range of cell lines
> Does not require removal of serum or culture medium
> Does not require washing or changing of medium after transfection
> Low cytotoxicity
BHK-21, HepG2, CHO, K562, HeLa, NIH 3T3
Lipofectamine 2000
GreenFect™ > Superior transfection efficiency for difficult-to-transfect cells
> Does not require removal of serum or culture medium
> Does not require washing or changing of medium after transfection
> Improved cell viability: Gentle on your cells with low toxicity
Hard-to-transfect cells
Lipofectamine 3000

Case Studies

Figure A & B. Assessment of PrimeFectTM transfection efficiencies for siRNA. Hela cell line was transfected with either a GFP cDNA (A), or co-tranfected with GFP cDNA and an anti-GFP siRNA (B). Transfection efficiencies were assessed by GFP signal.  
Figure C & D. Comparison of PrimeFectTM and Lipofectamine® 2000 in plasmid transfection on mouse neuronal cells. Mouse neuronal cells were transfected with a GFP-expressing plasmid using either PrimeFect™ transfection reagent (C), or Lipofectamine® 2000 (D). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter). 

Assessment of GreenFect™ transfection efficiencies using GFP visualization. A549, NIH3T3, HepG2, and MCF7 cell lines were transfected with a GFP-expressing plasmid using the indicated transfection reagents. Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).

Product Name
Cat. #
Price View
PeneFect™ Transfection Reagent (1 ml) M0001-01 $149
LiFect293™ Transfection Reagent (1 ml) M0002-01 $199
LiFectRNA™ Transfection Reagent (1 ml) M0141 $249
GreenFect™ Transfection Reagent (0.75 ml) M0004-01 $299
PrimeFect™ Transfection Reagent (1 ml) M0003-01 $199


1. Intranasal pediatric parainfluenza virus-vectored SARS-CoV-2 vaccine is protective in monkeys. 
Publication: Cell        Product: LiFect293™ Transfection Reagent

2. Increased neutralization potency and breadth elicited by a SARS-CoV-2 mRNA vaccine forming virus-like particles. 
Publication: PNAS        Product: LiFect293™ Transfection Reagent

3. A multiclade env–gag VLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques. 
Publication: Nature Medicine        Product: LiFect293™ Transfection Reagent

4. Live-attenuated pediatric parainfluenza vaccine expressing 6P-stabilized SARS-CoV-2 spike protein is protective against SARS-CoV-2 variants in hamsters. 
Publication: PLOS        Product: LiFect293™ Transfection Reagent

5. Functional Anatomy of the Trimer Apex Reveals Key Hydrophobic Constraints That Maintain the HIV-1 Envelope Spike in a Closed State. 
Publication: ASM        Product: LiFect293™ Transfection Reagent


"We are happy to share with you that the sample given to us has worked very good. The results are really satisfactory. We would like to shift totally towards LifeSct from Lipofectamine and Fugene for our experiments in future."

------ India THSTI, Tripti Shrivastava, PHD

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