|Key Features:||1. Bio-degradable after endocytosis
2. Exceptional high titers of virus production
3. Equally good for very long DNAs (>89 kb)
4. Equally good for both single DNA transfection and multi DNA co-transfection
5. High levels of recombinant protein production
6. Simple & robust transfection procedure
7. Very affordable
||Based on our proprietary polymer synthesis technology, PeneFect™ Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. PeneFect™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis , leading to much less cytotoxicity. PeneFect™ reagent, 1.0 ml, is sufficient for ~667 transfections in 24 well plates or ~333 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.
||1. PeneFect™ Transfection Reagent: 1 ml|
||PeneFect™ Transfection Reagent (4×100 µl, $79)
PeneFect™ Transfection Reagent (4 ml, $529)
Protocol for Suspension 293 and CHO Cells
Protocol for Lentivirus Production
Protocol for rAAV Production
A comparison showing transfection efficiency of PeneFect™ reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells. HEK-293T cells were transfected with GFP vector (pEGFP-N3) by PeneFect™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection
A comparison showing transfection efficiency of PeneFect™ reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by PeneFect™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.