Transfection Reagents

Transfection Reagents

Based on our unique polymer synthesis technologies, currently we are developing and manufacturing four categories of DNA transfection reagents, which show distinct transfection characteristics compared with the leading products in the market. What's more important is that the transfection reagents we are manufacturing are very affordable and will save your precious research budget.

Selection Guide

Reagents
Features
Sample Type
Cell Type
Replacement
LiFect293TM > Best for suspension 293 cells 
> High levels of recombinant protein production
> Presence of serum and antibiotics enhances efficiency on 293 cells
> Exceptional efficiency for both single DNA transfection and multi DNAs co-transfection
> Exceptional high titers of virus production
DNA
HEK293 related cells
292Fectin, Fugene 6
PrimeFectTM > Superior transfection efficiency for a broad range of cell lines
> Does not require removal of serum or culture medium
> Does not require washing or changing of medium after transfection
> Low cytotoxicity
DNA, RNA BHK-21, HepG2, CHO, K562, HeLa, NIH 3T3
Lipofectamine 2000
GreenFectTM > Superior transfection efficiency for difficult-to-transfect cells
> Does not require removal of serum or culture medium
> Does not require washing or changing of medium after transfection
> Improved cell viability: Gentle on your cells with low toxicity
> Suitable for high-throughput application
DNA, RNA
DNA/RNA
Hard-to-transfect cells
Lipofectamine 3000
PeneFectTM > Excellent for both single DNA transfection and 
   multi DNA co-transfection

> Simple & robust transfection procedure
> Cost-effective

DNA

Mammalian cells

Lipofectamine 2000

Case Study


A comparison of LiFect293TM reagent vs. 293fectin, Xfect and Fugene 6 on protein production with suspension 293F cells. 30 ml of 293F cell cultured in standard culture medium was transfected with pEGFP-6xHis plasmid using LiFect293TM In Vitro DNA Transfection Reagent (20 µg plasmid DNA), 293Fectin (30 µg plasmid DNA), Xfect (30 µg plasmid DNA) and Fugene 6 (30 µg plasmid DNA) per manufacturers' standard transfection protocols. GFP fluorescence was visualized 48 hours post transfection (left panel) with A for LipoD293, B for 293fectin, C for Xfect and D for Fugene 6. The 6xHis tagged GFP protein was then purified via Ni-NTA affinity column. 5 µl of 1st elution fraction was resolved on SDS-PAGE followed by Coomassie Brilliant Blue staining (right upper panel E) with the lane 1 for LipoD293, lane 2 for protein marker, lane 3 for Xfect, lane 4 for 293fectin and lane 5 for Fugene 6. The protein yield was quantified via spectrometer (right lower panel F). Learn more >>


Figure A & B. Assessment of PrimeFectTM transfection efficiencies for siRNA. Hela cell line was transfected with either a GFP cDNA (A), or co-tranfected with GFP cDNA and an anti-GFP siRNA (B). Transfection efficiencies were assessed by GFP signal.  
Figure C & D. Comparison of PrimeFectTM and Lipofectamine® 2000 in plasmid transfection on mouse neuronal cells. Mouse neuronal cells were transfected with a GFP-expressing plasmid using either PrimeFect™ transfection reagent (C), or Lipofectamine® 2000 (D). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).


A comparison showing transfection efficiency of PeneFectTM reagent vs. Lipofectamine 2000 on HepG2 cells.HepG2 cells were transfected with GFP vector (pEGFP-N3) by PeneFectTM (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection. Learn more >> 

Product Name
Cat. #
Size
Price Order
GreenFectTM Transfection Reagent M0004-01 0.75 ml
$299
PrimeFectTM Transfection Reagent M0003-01 1 ml
$199
PrimeFectTM Transfection Reagent for Hela Cells M0003-HC 1 ml
$199
PrimeFectTM Transfection Reagent for CHO Cells M0003-CC 1 ml
$229
PrimeFectTM Transfection Reagent for HepG2 Cells M0003-EC 1 ml
$229
PrimeFectTM Transfection Reagent for BHK-21 Cells M0003-BC 1 ml
$199
PrimeFectTM Transfection Reagent for K562 Cells M0003-KC 1 ml
$289
LiFect293TM Transfection Reagent M0002-01 1 ml
$199
PeneFectTM Transfection Reagent M0001-01 1 ml
$149

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