LiFluor™ 405 is a blue fluorescent dye spectrally similar to Pacific Blue dye. However, compared to Pacific Blue dye, LiFluor™ 405M dye is more bright, and more photostable. The dye can be used as a replacement for Pacific Blue, BD Horizon V450, eFluor 450.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting LiFluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Key Features
• LiFluor™ fluorescent dye has superior brightness and good photostability.
• The dye is insensitive to pH change and has good water solubility.
• Multiplexing: across spectrum from UV to near-IR.
Specifications
1. Ex/Em: 405/450 nm
2. Extinction coefficient: 40,000
3. Molecular weight: ~500
4. Excitation source: 405 nm
Applications
Fluorescent labeling
Component
1. LiFluor™ 405M Fluorescent Dye: 5 mg
Storage
Store at -20°C and protect from light.
Images
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Immunofluorescent stain of α-tubulin with LiFluor™ 405M conjugated Goat Anti-Mouse IgG (H+L) Antibody |
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Immunofluorescent stain of α-tubulin with LiFluor™ 405M conjugated Goat Anti-Rabbit IgG (H+L) Antibody |
