LiFluor™ 555 is a bright, orange-red fluorescent dye spectrally similar to Cy3 dye. However, compared to Cy3, LiFluor™ 555 dye is much brighter and more photostable. The dye can be used as a replacement for Alexa Fluor 555, DyLight 549, Cy3, TAMRA.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting LiFluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Key Features
• LiFluor™ fluorescent dye has superior brightness and good photostability.
• The dye is insensitive to pH change and has good water solubility.
• Multiplexing: across spectrum from UV to near-IR.
Specifications
1. Ex/Em: 556/575 nm
2. Extinction coefficient: 150,000
3. Molecular weight: ~1,000
4. Excitation source: 555 nm
Applications
Fluorescent labeling
Component
1. LiFluor™ 555 Fluorescent Dye: 5 mg
Storage
Store at -20°C and protect from light.
Images
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Immunofluorescent analysis of α-tubulin and CCSP with LiFluor™ 555 conjugated Goat Anti-Mouse IgG (H+L) Antibody. |
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Immunofluorescent analysis of α-tubulin and CCSP with LiFluor™ 555 conjugated Goat Anti-Rabbit IgG (H+L) Antibody |
