LiFluor™ 680 is a bright, near-IR dye that is spectrally similar to the Cy5.5 dye. LiFluor™ 680 dye is water-soluable, highly fluorescent and pH-insensitive from pH 4 to pH 10. The dye can be used as a replacement for Alexa Fluor 680, DyLight 680, Cy5.5, IR Dye 680.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting LiFluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Key Features
• LiFluor™ fluorescent dye has superior brightness and good photostability.
• The dye is insensitive to pH change and has good water solubility.
• Multiplexing: across spectrum from UV to near-IR.
Specifications
1. Ex/Em: 650/666 nm
2. Extinction coefficient: 240,000
3. Molecular weight: ~1,300
4. Excitation source: 633 nm
Applications
Fluorescent labeling
Component
1. LiFluor™ 680 Fluorescent Dye: 5 mg
Storage
Store at -20°C and protect from light.
Images
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Immunofluorescent analysis of α-tubulin with LiFluor™ 680 conjugated Goat Anti-Mouse IgG (H+L) Antibody. |
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Immunofluorescent analysis of α-tubulin with LiFluor™ 680 conjugated Goat Anti-Rabbit IgG (H+L) Antibody. |
