PhosphoFluor™ protein Gel Stain is a high sensitive fluorescent stain designed for selectively detecting phosphoproteins in polyacrylamide gels. This stain contains a PhosphoFluor™ group, which allows direct, in-gel detection of phosphate groups attached to tyrosine, serine, or threonine residues, without the need for antibodies or radioisotopes. The stain can be used with standard SDS-polyacrylamide gels or with 2-D gels.
Key Features
• High sensitivity. Detect as little as 1 ng phosphoprotein.
• Simple and fast staining.
• Compatibility with standard laboratory equipment.
• Wide linear detection range. At least three orders of magnitude.
• Compatible with downstream analysis: Compatible with MS and sequencing.
• Stable at room temperature for 1 year.
Applications
Protein gel stain
Component
1. PhosphoFluor™ protein Gel Stain: 500 ml
Storage
Store at 2-25°C and protect from light.
Case Study
Gel stain results with Coomassie® Blue Fast Stain (left), SuperLumin™ Protein Gel Stain (middle), and PhosphoFluor™ Protein Gel Stain, followed by total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.
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