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| Key Features: | • Rapid: DNA purification process in less than 20 min post-lysis. • Safe: No organic extraction, no ethanol precipitation. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: | LiPureTM Bacteria DNA Purification Kit provides a rapid and easy method for the isolation of high-quality genomic DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1× 109 bacterial cells can be processed for each column. The buffer system is optimized to allow direct cell lysis followed by selective binding of DNA to the DNA column membrane. Simple centrifugation processing completely removes contaminations and enzyme inhibitors such as proteins and divalent cations, and allows simultaneous processing of multiple samples in parallel. Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion. |
| Applications: | Genomic DNA isolation from gram-positive and negative bacterial cells. |
| Technical Specifications: | • Sample Input: 1 x 109 cells • Elution Volume: 50-100 μl • Technology: Silica spin column |
| Sample Source: | Bacterial cells |
| Component: | 1. Buffer ATL: 15 ml 2. Buffer DL: 15 ml 3. Buffer AW1: 15 ml 4. Buffer AW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. Lysozyme: 100 mg 8. Mini Column: 50 9. 2 ml Collection Tube: 50 |
| Storage: | Buffer & tube at RT; Proteinase K & lysozyme at -20°C |
| Documents: | Manual |
Case Study
Figure 1. Genomic DNA from E. coli cells was isolated using LiPureTM Bacteria DNA Purification Kit. Eluted DNA was analyzed on a 1% agarose gel.
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