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| Key Features: |
• Rapid: DNA purification process in less than 30 min. • Safe: No Phenol/chloroform extractions. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: |
LiPureTM Blood DNA Purification Kit provides rapid total DNA isolation from 10-250 µl of fresh and frozen anticoagulated whole blood. This kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extraction, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. The isolated DNA is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion. |
| Applications: |
Total DNA isolation from fresh and frozen anticoagulated whole blood. |
| Technical Specifications: |
• Sample Input: 10-250 μl • Elution Volume: 50-100 μl • Technology: Silica spin column |
| Sample Source: |
Fresh and frozen anticoagulated whole blood, buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and other body fluids. |
| Component: |
1. Buffer AL: 60 ml 2. Buffer DW1: 53 ml 3. Buffer DW2: 2×25 ml 4. Buffer AE: 30 ml 5. Mini Column: 200 6. 2 ml Collection Tube: 200 |
| Storage: |
At RT |
| Documents: |
Manual |
Case Study
Table 1. DNA yield and quality using LiPureTM Plasmid Mini Kit
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