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| Key Features: | • Rapid: DNA purification process in less than 30 min. • Safe: No organic extraction, no ethanol precipitation. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: | LiPureTM Cell DNA Purification Kit provides rapid total DNA isolation from cultured cells. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extraction, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. The isolated DNA is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion. |
| Applications: | Total DNA isolation from cultured cells. |
| Technical Specifications: | • Sample Input: 5 x 106 cells • Elution Volume: 50-100 μl • Technology: Silica spin column |
| Sample Source: | Cultured cells |
| Component: | 1. Buffer AL: 15 ml 2. Buffer DW1: 15 ml 3. Buffer DW2: 15 ml 4. Buffer AE: 15 ml 5. Mini Column: 50 6. 2 ml Collection Tube: 50 |
| Storage: | At RT |
| Documents: | Manual |
Case Study
Figure 1. Genomic DNA from different cell lines was isolated using LiPureTM Cell DNA Purification Kit from animal cells. Eluted DNA was analyzed on a 0.8% agarose gel.
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