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| Key Features: | • Rapid: DNA purification process in less than 20 min post-lysis. • Safe: No organic extraction, no ethanol precipitation. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: | LiPureTM Magbead Bacteria DNA Purification Kit provides a rapid and easy method for the isolation of high-quality genomic DNA from a wide variety of gram-positive and negative bacterial species. This kit can be processed manually or on an automated platform. The buffer system is optimized to allow direct cell lysis followed by selective binding of DNA to the magnetic beads. Three rapid wash steps remove trace salts and protein contaminants, and finally, DNA is eluted in water or low ionic strength buffer. Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion. |
| Applications: | Genomic DNA isolation from gram-positive and negative bacterial cells. |
| Technical Specifications: | • Sample Input: 1 x 109 cells • Elution Volume: 50-100 μl • Technology: Magnetic Beads |
| Sample Source: | Bacterial cells |
| Component: | 1. Buffer ATL: 15 ml 2. Buffer DL: 15 ml 3. Buffer AW1: 15 ml 4. Buffer AW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. Lysozyme: 100 mg 8. MagBinding Beads: 2.5 ml |
| Storage: | Buffer & tube at RT; Proteinase K & lysozyme at -20°C |
| Documents: | Manual |
Case Study
Figure 1. Genomic DNA from various bacteria was isolated using LiPureTM Magbead Bacteria DNA Purification Kit. Eluted DNA was analyzed on a 1% agarose gel.
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