Recombinant Anti-beta III Tubulin antibody (HRP Conjugated)  (100 μl)

Recombinant Anti-beta III Tubulin antibody (HRP Conjugated) (100 μl)

Brand: ServiceBio
Cat. #: ZB15139-HRP-100
Availability: In Stock
$259.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB    
Gene Name: Tubulin beta-3 chain
Synonyms:

TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin, TUJ-1,TUJ 1, TUJ1, tuj1

Uniprot ID: Q9ERD7
Immunogen: KLH conjugated synthetic peptide corresponding to Mouse beta III- Tubulin
Isotype: IgG1
ConjugationHRP
Purity: Affinity purification
Documents:Manual-TUBB3 Antibody




Product Usage Information

Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 1000-1: 2000

U-251 MG, SH-SY5Y, brain

Incubation condition

After blocking, incubate the membrane with HRP conjugated Anti-COX IV antibody (ZB15250-HRP) for 3-4 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.




Background

Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response. This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.




Images

TUBB3 antibody

WB analysis of beta III Tubulin (ZB15139-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Antibody: HRP conjugated anti-beta III Tubulin antibody 1: 2000, 4°C overnight.





Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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