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Recombinant Anti-beta III Tubulin antibody (HRP Conjugated) (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | Tubulin beta-3 chain |
Synonyms: | TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin, TUJ-1,TUJ 1, TUJ1, tuj1 |
Immunogen: | KLH conjugated synthetic peptide corresponding to Mouse beta III- Tubulin |
Uniprot ID: | Q9ERD7 |
Isotype: | IgG1 |
Conjugation | HRP |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 55 kDa / 55 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | U-251 MG, SH-SY5Y, brain |
Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-beta III Tubulin antibody(ZB15139-HRP) for 3-4 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST | ||
Background
Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.
Images
| WB analysis of beta III Tubulin(ZB15139-HRP). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-beta III Tubulin antibody 1: 2000, 4°C overnight. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |