LiFluor™ Fluorescent Dyes

LiFluor™  Fluorescent Dyes

Fluorescent labeling of biomolecules enables researchers to detect specific components of complex biological assemblies with exquisite sensitivity in applications ranging from imaging and flow cytometry, to Western blots. ABP Biosciences offers a full spectrum of LiFluor™ Dyes for labeling antibodies, proteins, peptides, ligands, synthetic oligonucleotides and other biomolecules for use with immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, and cytochemistry applications as well as probing biological structure, function and interactions. LifeSct offers a series of superior bright, highly photo-stable and excellent water-soluble LiFluor™ dyes spanning the visible and near-infrared (IR) spectrum for labeling biomolecules, especially proteins and nucleic acids.

Key Features

• LiFluor™ fluorescent dyes have superior brightness and good photostability
• The dyes are insensitive to pH change and has good water solubility
• Multiplexing: across spectrum from UV to near-IR

Fluorescent DyeEx/Em (nm)Laser TypeAdvantagesReplacement
LiFluor™ 350 Blue350/440UVHighly fluorescent; Highly water-
soluble and pH insensitive
AMCA, Alexa Fluor 350,
DyLight 350
LiFluor™ 405 Blue405/450405 nm laserBrighter than Pacific Blue; Less
spill-over in the green channel
Pacific Blue,
BD Horizon V450
LiFluor™ 405 Green412/500405 nm laserHighly fluorescent; Less spill-
over in the green channel
BD Horizon V500
LiFluor™ 488 Green495/520488 nm laserExtremely photostable; Highly
fluorescent and water-soluble
Alexa Fluor 488,
DyLight 488, Cy2, FITC
LiFluor™ 546 Red556/575532, 543, 546 or
555 nm laser
Extremely photostable;
Highly fluorescent and water-soluble
Alexa Fluor 546,
Cy3, TAMRA
LiFluor™ 555 Red553/565532, 543, 546 or
555 nm laser
Brighter than TAMRA; Highly
photostable and water-soluble
Alexa Fluor 555,
DyLight 549, Cy3, TAMRA
LiFluor™ 568 Red578/602543, 546, 555 or
568 nm laser
Highly water-soluble and
pH insensitive; Highly fluorescent
Alexa Fluor 568,
ATTO 565, Rhodamine Red
LiFluor™ 594 Red590/615555 or 568 nm
laser
Brighter than Texas Red; Highly
water-soluble and pH insensitive
Alexa Fluor 594,
DyLight 594, Texas Red
LiFluor™ 610 Red610/625555 or 568 nm
laser
Highly fluorescent; Highly water-
soluble and pH insensitive
Alexa Fluor 610
LiFluor™ 635 Red630/650633 or 635 nm
laser
Highly fluorescent; Highly water-
soluble and pH insensitive
Alexa Fluor 635,
DyLight 633
LiFluor™ 647 Red650/666633 or 635 nm laserBrighter than Cy5; Highly water-
soluble and pH insensitive
Alexa Fluor 647,
DyLight 649, Cy5
LiFluor™ 680 Red680/700680 or 685 nm laserBrighter than Cy5.5; Highly
water-soluble and pH insensitive
Alexa Fluor 680,
DyLight 680, Cy5.5
LiFluor™ 750 Red755/770680 or 685 nm laserHighly fluorescent; Highly water-
soluble and pH insensitive
Alexa Fluor 750,
DyLight 750, Cy7

Case Study


Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates of LiFluor™ dyes and Cy dyes at different dye:protein ratios in the conjugate.


Comparison of the photobleaching rates of LiFluor™ 488 goat anti-mouse IgG antibody conjugate (top) with FITC goat anti-mouse IgG antibody conjugate (bottom).

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