|Key Features:||• The procedure is fast and simple, amenable to automation. The entire process takes less than 1 hour.
• Does not use hazardous chemicals such as phenol, chloroform, proteinase K or guanidine isothiocyanate.
• There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA.
||LiDirectTM Lightning Genotyping kit is a combination of LiDirectTM Genotyping DNA Extraction Buffer and LiTaqTM Plus PCR master mix. It is designed for fast extraction of tissue DNA and subsequent PCR analysis. LiDirectTM Genotyping kit contains all the reagents needed to rapidly (15 min) extract genomic DNA from mouse tails, yeast, plant cells and other tissues, cells, hair shafts, and saliva. It also include all reagents for subsequent genotyping PCR. The whole genotyping process can be completed within 1 hour.|
||1. LiDirectTM Extraction Buffer: 150 ml
2. LiDirectTM Stop Solution: 250 ml
3. 2× LiTaqTM Plus PCR Master Mix: 25×1 ml
||PCR Master Mix: -20°C; Extraction Buffer & Stop Solution: 4-25°C
||LiDirectTM Lightning Genotyping Kit (400 rxn, $139)|
"The LiDirectTM Lightning Genotyping Kit that I sampled gave clear results and was much more efficient than the methods my lab had been using in the past. With the buffer that is included in the kit, there is no need to make an extraction buffer. Additionally, the quick extraction step (just 15 minutes) really cuts down on time and makes a huge difference. I am very satisfied with the product and plan on using this kit. Thank You!"
"I would like to update you on the LiDirectTM Lightning Genotyping Kit that I have been using for the past 6 month. We have definitely benefited from using this kit. The gel-electrophoresis-ready LiTaqTM Plus PCR Master Mix significantly cuts down the required hands-on time to genotype our animals. For a project that is heavily relied on multiple lines of transgenic animal, this has had a huge impact. Thank you for a wonderful product!"
"Using the LiDirectTM Lightning Genotyping Kit from your company, our lab members got pretty good results for positive control probes but not our mouse strains. The kit from your company is pretty good. We are optimizing the probe sequences and PCR conditions for genotyping now and at the meantime we are still using company service for genotyping. Once we are able to genotype our mouse strains in house, we will order LiTaqTM Plus PCR Master Mix from you."
Taking advantage of our new proprietary formula, the LiDirectTM DNA extraction buffer enables fast DNA release from the toughest cells, and performs much better than traditional alkaline lysis protocols. Combined with our industry leading LiTaqTM Plus DNA polymerase, our LiDirectTM Lightning Genotyping Kit helps scientists to genotype all types of tissues and cells effortlessly.
|Step 1: sample preparation
• 1~2 mm piece of mouse tail
• 5~10 mg of tissue
• ~1×104 cells
Step 2: add 75 µl of LiDirectTM Extraction Buffer
• Incubate at 98°C for 15 min
Step 3: add 125 µl of LiDirectTM Stop Solution
Step 4: set up 25 µl of LiTaqTM Plus PCR reaction mix
|PCR cycling for amplification:
1. 95°C: 15 sec
2. 35 ×
• Denaturation: 5 sec at 98°C
• Annealing: 10 sec at 55°C
• Extension: 30 sec/kb at 72°C
3. Final extension: 5 min at 72°C
From these results, it can be concluded that our LiDirectTM DNA extraction buffer is compatible with most Taq polymerases on the market, but our LiTaqTM plus product line is more robust than most other products on market.
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