1. Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.
2. Stable at room temperature.
3. Compatible with standard blue LED and UV transilluminators.
4. Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
LiGreenTM DNA Gel Stain is a stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. LiGreenTM is compatible with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader equipped with visible light excitation such as a 488 nm laser-based gel scanner. LiGreenTM DNA Gel Stain, 10,000× is a concentrated LiGreenTM solution that can be diluted 10,000 times for use in precast gel staining or 5,000 times for use in post gel staining according to the procedures described below. One vial of 10,000× solution can be used to prepare at least 100 precast minigels or post-stain at least 100 minigels.
Gel staining with LiGreenTM is compatible with downstream applications such as gel extraction and cloning. LiGreenTM is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
|Component::||1. LiGreenTM DNA Gel Stain (10,000× in water): 2× 500 μl|
|Storage:||Store at 2-25°C and protect from light.|