LiGreen™ Plus Nucleic Acid Gel Stain (500 μl)

LiGreen™ Plus Nucleic Acid Gel Stain (500 μl)

Cat. #: M0050-05
Availability: In Stock
$99.00
-+
Key Features: 

1. Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.

2. Stable at room temperature.

3. Compatible with standard blue LED and UV transilluminators.

4. Use with blue light reduces mutagenesis for gel excision and subsequent cloning.

Description:  

LiGreenTM Plus Nucleic Acid Gel Stain is a highly sensitive fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. This single stain gives high sensitivity detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or alternatively the stain can be added to agarose gels during gel casting. LiGreenTM Plus is compatible with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader equipped with visible light excitation such as a 488 nm laser-based gel scanner. 

Gel staining with LiGreenTM Plus is compatible with downstream applications such as gel extraction and cloning. LiGreenTM Plus is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Component:: 1. LiGreen™ Plus Nucleic Acid Gel Stain (10,000× in DMSO): 500 μl
Storage: Store at 2-25°C and protect from light.
Other Size:
LiGreenTM Plus Nucleic Acid Gel Stain (Free Sample)
Download:
Manual

Case Study


Gel stain results with LiGreenTM, LiGreenTM Plus, and LiGreenTM Ultra (Left), and comparison results with SYBRTM Safe and GelGreenTM (Right). Left: Two-fold serial dilutions of a 1 kb DNA ladder were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with LiGreenTM, LiGreenTM Plus, and LiGreenTM Ultra. Right: Results show LiGreenTM has similar sensitivity with SYBRTM Safe and GelGreenTM, LiGreenTM Plus and LiGreenTM Ultra have much better sensitivity.


Cell permeability tests with SYBRTM Safe, LiGreenTM, LiGreenTM Plus, and LiGreenTM Red. Hela cells were incubated at 37ºC with 1× SYBR Safe, LiGreenTM, LiGreenTM Plus, and LiGreenTM Red for 30 min. LiGreenTM Plus and LiGreenTM Red could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBRTM Safe, which rapidly entered cells and stained nuclei.

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