LiTaq™ PCR Master Mixes

LiTaq™ PCR Master Mixes

LiTaq™ DNA Polymerase is a thermostable DNA polymerase that exhibits a 5'→3 ' polymerase activity and a 5'→3 ' exonuclease activity, with no 3' →5 ' exonuclease activity. LiTaq™ DNA Polymerase is purified from an Escherichia coli (E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this enzyme.

2× LiTaq™ PCR Master Mix (M0024) is an optimized ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2, KCI and stabilizers. 2× LiTaq™ Plus PCR Master Mix (M0025) is a mixture of LiTaq™ plus DNA polymerase, dNTP, an optimized buffer system and an enzyme containing 3’→5’ exonuclease activity. Its fidelity is 6 times greater than that of 2× LiTaq™ Master Mix. LiTaq™ PCR mix and LiTaq™ Plus PCR mix are very cost-effective and ideal for genotyping. 

PCR Master Mix

Case Study

Samples: Human genomic DNA & cDNA, Mouse genomic DNA, Wheat genomic DNA, Rice genomic DNA, Coli colony

LifeSct: LiTaq™ PCR mix, LiTaq™ DNA polymerase, 2× LiTaq™ plus PCR mix, 2× LiTaq™ Eco PCR mix

Competitors: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F

PCR reaction setup for amplification:
• 2× Mix: 12.5 µl
• F1: 1 µl
• R1 : 1 µl
• Template* : 0.5 µl
• ddH2O: 0.2 ul
* gDNA: 50 ng; λ DNA: 5 ng; Plasmid: 2 ng;  Colony: 1 µl

PCR cycling for amplification:
1. 95°C: 3 min
2. 35 x
    1. Denaturation: 15 s at 95°C
    2. Annealing: 20 s at 60°C
    3. Extension: 1 min/kb at 72°C
3. Final extension: 5 min at 72°C

From the above results, it can be concluded that for PCR amplification of different length and kind of templates, all four PCR mixes from our company performed superbly. For general PCR, our products have the broadest applicability, best success rate, highest specificity and maximal yield. In the most difficult situations, when all other PCR mixes failed, at least one of our products still worked.

Product Name
Cat. #
Price View
2× LiTaq™ PCR Master Mix (+Dye) (5×1 ml) M0024-05 $99
2× LiTaq™ PCR Master Mix (-Dye) (15×1 ml) M0024-03 $229
2× LiTaq™ Plus PCR Master Mix (+Dye) (5×1 ml) M0025-05 $139
2× LiTaq™ Plus PCR Master Mix (-Dye) (15×1 ml) M0025-03 $349
2× LiTaq™ HotStart PCR Master Mix (+Dye) (5×1 ml) M0021-01 $159
2× LiTaq™ HotStart PCR Master Mix (-Dye) (5×1 ml) M0021-02 $159
2× LiTaq™ SuFi PCR Master Mix (1 ml) M0031 $129

Customer Testimonials

"Our lab started to use the high-quality services from LifeSct, including the gene synthesis and the gBlocks gene fragments since 2017. We are always happy with the great quality of the services, the reasonable price, the prompt shipment and the good communication from LifeSct. More impressively, your LiTaq™ PCR Master Mix is robust and cost-effective. Thanks for continuing to produce great products and provide excellent services!"

------ NIH/NICHD, Dr. Chune Cao, Lab manager

"I'm citing 2× LiTaq™ Plus PCR Master Mix (LifeSct LLC) in my manuscript for a protein engineering paper. It is extremely effective, and I really appreciated the sample. I'll send you a copy as soon as it's published.BTW, LiScript™ Fast RT Master Mix works great, actually I wanted to mention how fast and easy it was to use. The addition of RNAse inhibitor and the 5x concentration are perfect for fast experiments."

------ San Diego State University, Dr. Brent White, Postdoc

"I have been using LiTaq™ Plus PCR Master Mix and LiQuant™ Universal Green qPCR master mix in my experiments for two years. The PCR mix can impressively amplify the difficult sequences in my hands. Amazingly, it rapidly amplifies the templates at ~ 2kb/min. And the qPCR mix has the higher sensitivity compared with the similar product from other company. I highly recommend these products!!!"

------ UPENN, Dr. Janne Liu, Research Associate

"Our Lab had a chance to try the LiTaq™ Plus PCR Master Mix and LiClone™ One Step DNA Assembly Kit which perform very well compared with similar products we used before. We will order them later. Thank you!"

------ NDSU, Dr. Ang Guo, Assistant Professor

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