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LiGreen™ Nucleic Acid Gel Stains
LiGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. These stains are next-generation fluorescent nucleic acid gel stains designed to replace the highly mutagenic ethidium bromide (EB). These dyes are superior to EB by having a combination of high sensitivity, low toxicity and great stability.
Key Features
• Safer than EB: Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.
• Ultra-sensitive: Much more sensitive than GelGreen and SYBR Safe.
• Compatible with standard blue LED and UV transilluminators.
• Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
Customer Testimonials
"I have been using LiGreen™ Ultra Nucleic Acid Gel Stain and dNTPs set (100mM each) more than one years, both products are of excellent quality and are cheaper than other companies, I will continue to use them."
------ NIH/NCI, Dr. Ping Yu, Staff Scientist
| LiGreen™ | LiGreen™ Plus | LiGreen™ Ultra | LiGreen™ Red | |
| Sensitivity (dsDNA) | Sensitive (~2 ng) | Highly sensitive (~0.2 ng) | Ultra sensitive (~60 pg) | Sensitive (~1 ng) |
| Nonhazardous | √ | √ | √ | √ |
| dsDNA | √ | √ | √ | √ |
| ssDNA | √ | √ | ||
| RNA | √ | √ | ||
| Precast gel staining | √ | √ | √ | |
| Post-gel staining | √ | |||
| Gel band excision for cloning | √ | √ | √ | |
| Excitation Source | Blue light/UV | Blue light/UV | Blue light/UV | UV |
| Recommended emission filter | SYBR filter | SYBR filter | SYBR filter | EB filter |
| Product Name | Cat. # | Features | Request |
| LiGreen™ Plus Nucleic Acid Gel Stain | M0050 | High sensitivity, stability & low toxicity | Free Product |
| LiGreen™ Ultra Nucleic Acid Gel Stain | M0051 | Ultra sensitivity, stability & low toxicity | Free Product |
Case Study
Gel stain results with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra (Left), and comparison results with SYBR Safe and GelGreen (Right). Left: Two-fold serial dilutions of a 1 kb DNA ladder were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra. Right: Results show LiGreen™ has similar sensitivity with SYBR Safe and GelGreen, LiGreen™ Plus and LiGreen™ Ultra have much better sensitivity.
Cell permeability tests with SYBR Safe, LiGreen™, LiGreen™ Plus, and LiGreen™ Red. Hela cells were incubated at 37ºC with 1× SYBR Safe, LiGreen™, LiGreen™ Plus, and LiGreen™ Red for 30 min. LiGreen™ Plus and LiGreen™ Red could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe, which rapidly entered cells and stained nuclei.
| Product Name | Cat. # | Size | Price | Order |
| LiGreen™ DNA Gel Stain | M0049 | 1 ml | $149 |  |
| LiGreen™ Plus Nucleic Acid Gel Stain | M0050-05 | 500 µl | $119 | |
| LiGreen™ Plus Nucleic Acid Gel Stain | M0050-20 | 4×500 µl | $369 | |
| LiGreen™ Ultra Nucleic Acid Gel Stain | M0051 | 500 µl | $149 | |
| LiGreen™ Red DNA Gel Stain | M0054 | 500 µl | $99 | |
| LiGreen™ Landing Dye | M0052 | 2 ml | $99 | |
| LiGreen™ Red Landing Dye | M0055 | 2 ml | $99 | |
Related Products
| Product Name | Cat. # | Size | Price | |
| LiDirect™ Lightning Genotyping Kit | M0015 | 400 rxn | $139 | Read more |
| 2× LiTaq™ PCR Master Mix | M0024 | 15 ml | $189 | Read more |
| 2× LiSpark™ Ultra SuFi PCR Master Mix | M0031 | 1 ml | $129 | Read more |
| LiQuant™ Green qPCR Master Mix | M0026 | 500 rxn | $199 | Read more |
| LiQuant™ NGS dsDNA Assay Kit | M0066 | 500 rxn | $349 | Read more |
| dNTP Set (100 mM each) | M0059 | 4×250 µl | $129 | Read more |
