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DNA topoisomerase I (TOP1) Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | DNA topoisomerase I |
| Gene Symbol: | TOP1 |
| Synonyms: | TOPI; DNA topoisomerase I (TOP1) |
| Gene ID: | 7150 |
| UniProt ID: | P11387 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (DNA topoisomerase I (TOP1)) (NP_003277.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.17 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-TOP1 antibody |
Background
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Images
 | Western blot analysis of various lysates using DNA topoisomerase I (TOP1) Rabbit pAb (A12524) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse liver using DNA topoisomerase I (TOP1) Rabbit pAb (A12524) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A12524). Western blot was performed from the immunoprecipitate using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A12524) at a dilution of 1:1000. |
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