2× LiTaq™ Ultra HiFi PCR Master Mix (1 ml)

LiTaq™ Ultra HiFi PCR Master Mix is a ready-to-use super-fidelity PCR master mix designed for superior accuracy and performance. This high fidelity PCR master mix incorporates a genetically engineered HotStart high fidelity DNA polymerase, delivering exceptional DNA affinity and processivity. It excels with complex and partially degraded templates, offering a fidelity >100× higher than routine taq polymerase. Enhanced with unique extension and specificity-promoting factors, this hifi PCR master mix significantly boosts long-fragment amplification capability, specificity, and product yield.
 

Key Features

• Unmatched Fidelity: >100× lower error rate than routine taq DNA polymerase (Ultra High-Fidelity).

• Superior Performance: Robust amplification of fragments up to 20kb with minimal optimization.

• High Efficiency: Rapid extension rate (up to 10 sec/kb) delivers high product yield using minimal enzyme.

• Template Versatility: Ideal for routine PCR, long fragments, difficult templates, and GC-rich templates (no buffer change needed).

• Exceptional Specificity & Yield: Outperforms most market polymerases for cleaner, more abundant results.
 

Applications

• High fidelity PCR

• High yield and fast PCR

• Blunt end cloning

• Complex templates

• GC-rich PCR

• Site-directed mutagenesis
 

Component

1. 2× LiTaq™ Ultra HiFi PCR Master Mix: 1 ml
 

Storage

Store at -20°C.
 

Case Study

Samples: Human Genomic DNA & cDNA, Blood Samples, Mouse Tail Lysate

Product: LiTaq™ Ultra HiFi PCR Master Mix

From the above results, it can be concluded that LiTaq™ high fidelity PCR mix is suitable for the amplification of a variety of difficult templates. Under high GC, low template concentration and high inhibitor conditions, LiTaq™ DNA polymerase invariantly performed better than other polymerases. Although direct amplification of lysates is common practice for shorter fragments, amplification of longer fragments is more consistent with LiTaq™ DNA polymerase.