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Key Features: | • Bright signals with low background • Wide variety of fluorophores • Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more |
Description: | 5-Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as a 10 mM solution in TE buffer. 5-Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP. |
Specifications: | 1. Molecular Formula: C12H17N3Na3O14P3 2. Molecular Weight: 589.17 |
Applications: | Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension. |
Component: | 1. 5-Aminoallyl-dUTP: 100 μl, 10 mM in TE buffer |
Storage: | At -20°C |
Download: | Manual |