SuperECL™ Western Blotting Detection Kits

SuperECL™ Western Blotting Detection Kits

LifeSct offers two types of super sensitive chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP). The signal from ECL substrates can be captured and documented using X-ray film or a capable CCD camera imager.

Key Features

• High sensitivity: detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane.
• High signal stability: incubated blots provide stable signal duration over 4 hour time period.
• Stable reagent: reagent is stable for one year.
• Cost-effective: use much less antibody.

SensitivitySignal DurationPrimary Ab DilutionSecondary Ab Dilution
SuperECLTMPicogram to low femtogram< 8h1:1,000-1:30,0001:50,000-1:200,000
SuperECLTM PlusHigh Femtogram< 4h1:5,000-1:30,0001:100,000-1:300,000

Customer Testimonials

"We tested the SuperECL™ Western Blotting Detection Kit sample, and it did work well, same as the Pierce’s one. BTW, your LiPure™ Plasmid Mini Kit works very well, better than others we used. Also I have been trying to convince our purchasing department you are a good supplier to be considered to add to our vendor list.""

------ University of Pittsburgh, Dr. Qingde Wang, PI

"I have been using LifeSct’s SuperECL Western Blotting Detection Kit for a while and recently also start using LifeSct’s SuperECL Plus Western Blotting Detection Kit product. Both products work perfectly and the results are even better than pico and femto level products from the major brand I used before. I am very happy with these wonderful products."

------ NIH/NHLBI, Dr. Pingyuan Wang, PHD

Case Study

The comparison of SuperECLTM plus and SuperSignalTM (Thermo) on exposure time (Provided by Dr. Pingyuan Wang).

The comparison on exposure time(Left); The comparison on signal duration (Right).

The comparison of sensitivity of SuperECLTM, SuperECLTM Plus, and ImmobilonTM (Millipore) Western blot substrate (Left); The comparison on antibody dilution (Right).

Product Name
Cat. #
SuperECLTM Western Blotting Detection KitM0013-052×50 ml
SuperECLTM Western Blotting Detection KitM0013-252×250 ml
SuperECLTM Plus Western Blotting Detection KitM0053-052×50 ml
SuperECLTM Plus Western Blotting Detection KitM0053-102×100 ml

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