|Key Feature:|| |
• 5 minutes cDNA synthesis
• cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
• Passive reference dyes are provided for different qPCR instruments.
LiScript™ One Step Probe RT-qPCR Kit combines the first-strand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. LiScript™ Probe One Step RT-qPCR Kit contains all the necessary reagents for cDNA synthesis and qPCR except probe, total RNA/mRNA template and gene specific primers.
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• Viral RNA and trace RNA detection
1. RNase free ddH2O: 1.25 ml×2
2. 2× One Step Probe Mix: 1.25 ml×2
3. One Step Probe Enzyme Mix: 250 μl
4. 50× ROX Reference Dye 1: 100 μl
5. 50× ROX Reference Dye 2: 100 μl
|Storage:||At -20°C for one years|