2× LiTaq™ Plus PCR Master Mix (+Dye) (50 ml)

2× LiTaq™ Plus PCR Master Mix (+Dye) (50 ml)

Cat. #: M0025-50
Availability: In Stock
Key Features: 

• PCR products can be directly used for cloning into T-Vectors as most PCR products amplified with Taq DNA polymerase have one A at the 3'-terminus.

• Very low cost and ideal for genotyping.

2× LiTaqTM Plus Master Mix is a mixture of LiTaqTM DNA polymerase, dNTP, an optimized buffer system and an enzyme containing 3’→5’ exonuclease activity. Its fidelity is 6 times greater than that of Taq DNA Polymerase. Compared with Taq DNA Polymerase, LiTaqTM Plus DNA polymerase has stronger amplification performance, higher sensitivity, and is more tolerant of impurities within 5 kb amplifying range. It can amplify up to 10 kb from human genomic DNA or up to 15 kb from λDNA. Protective agents in the 2× Taq Plus Master Mix enable the resistance to repeated freeze-thaw cycles. The obtained PCR products are compatible with LiCloneTM One Step Cloning Kit (Cat. #: M0010), and can be directly used for cloning into T-Vectors as most PCR products amplified with Taq DNA polymerase have one A at the 3'-terminus.

Applications: • Routine PCR
• Genotyping
1. 2× LiTaqTM Plus Master Mix (+Dye): 50×1 ml
At -20°C
Other Size:
2× LiTaqTM Plus PCR Master Mix (+Dye) (5×1 ml, $169)
2× LiTaqTM Plus PCR Master Mix (+Dye) (15×1 ml, $389)

Customer Testimonials

"I'm citing 2× LiTaq™ Plus PCR Master Mix (LifeSct LLC) in my manuscript for a protein engineering paper. It is extremely effective, and I really appreciated the sample. I'll send you a copy as soon as it's published.BTW, LiScript™ Fast RT Master Mix works great, actually I wanted to mention how fast and easy it was to use. The addition of RNAse inhibitor and the 5x concentration are perfect for fast experiments."

------ San Diego State University, Dr. Brent White, Postdoc

"I have been using LiTaq™ Plus PCR Master Mix and LiQuant™ Universal Green qPCR master mix in my experiments for two years. The PCR mix can impressively amplify the difficult sequences in my hands. Amazingly, it rapidly amplifies the templates at ~ 2kb/min. And the qPCR mix has the higher sensitivity compared with the similar product from other company. I highly recommend these products!!!"

------ UPENN, Dr. Janne Liu, Research Associate

"Our Lab had a chance to try the LiTaq™ Plus PCR Master Mix and LiClone™ One Step DNA Assembly Kit which perform very well compared with similar products We used before."

------ NDSU, Dr. Ang Guo, Assistant Professor

Case Study

Samples: Human genomic DNA & cDNA, Mouse genomic DNA, Wheat genomic DNA, Rice genomic DNA, Coli colony

LifeSct: LiTaqTM PCR mix, LiTaqTM DNA polymerase, 2× LiTaqTM plus PCR mix, 2× LiTaqTM Eco PCR mix

Competitors: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F

PCR reaction setup for amplification:
• 2× Mix: 12.5 µl
• F1: 1 µl
• R1 : 1 µl
• Template* : 0.5 µl
• ddH2O: 0.2 ul
* gDNA: 50 ng; λ DNA: 5 ng; Plasmid: 2 ng;  Colony: 1 µl/p>

PCR cycling for amplification:
1. 95°C: 3 min
2. 35 x
    1. Denaturation: 15 s at 95°C
    2. Annealing: 20 s at 60°C
    3. Extension: 1 min/kb at 72°C
3. Final extension: 5 min at 72°C

From the above results, it can be concluded that for PCR amplification of different length and kind of templates, all four PCR mixes from our company performed superbly. For general PCR, our products have the broadest applicability, best success rate, highest specificity and maximal yield. In the most difficult situations, when all other PCR mixes failed, at least one of our products still worked.

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