• PCR products can be directly used for cloning into T-Vectors as most PCR products amplified with Taq DNA polymerase have one A at the 3'-terminus.
• Very low cost and ideal for genotyping.
||2× LiTaqTM Plus Master Mix is a mixture of LiTaqTM DNA polymerase, dNTP, an optimized buffer system and an enzyme containing 3’→5’ exonuclease activity. Its fidelity is 6 times greater than that of Taq DNA Polymerase. Compared with Taq DNA Polymerase, LiTaqTM Plus DNA polymerase has stronger amplification performance, higher sensitivity, and is more tolerant of impurities within 5 kb amplifying range. It can amplify up to 10 kb from human genomic DNA or up to 15 kb from λDNA. Protective agents in the 2× Taq Plus Master Mix enable the resistance to repeated freeze-thaw cycles. The obtained PCR products are compatible with LiCloneTM One Step DNA Assembly Kit (Cat. #: M0010), and can be directly used for cloning into T-Vectors as most PCR products amplified with Taq DNA polymerase have one A at the 3'-terminus.|
|Applications:||• Routine PCR
||1. 2× LiTaqTM Plus Master Mix (+Dye): 15×1 ml|
||2× LiTaqTM Plus PCR Master Mix (+Dye) (5×1 ml, $99)
2× LiTaqTM Plus PCR Master Mix (+Dye) (50×1 ml, $599)
"I have been using LiTaqTM Plus PCR Master Mix and LiQuantTM Universal Green qPCR master mix in my experiments for two years. The PCR mix can impressively amplify the difficult sequences in my hands. Amazingly, it rapidly amplifies the templates at ~ 2kb/min. And the qPCR mix has the higher sensitivity compared with the similar product from other company. I highly recommend these products!!!"
"Our Lab had a chance to try the LiTaqTM Plus PCR Master Mix and LiCloneTM One Step DNA Assembly Kit which perform very well compared with similar products we used before. We will order them later. Thank you!"
Samples: Human genomic DNA & cDNA, Mouse genomic DNA, Wheat genomic DNA, Rice genomic DNA, Coli colony
LifeSct: LiTaqTM PCR mix, LiTaqTM DNA polymerase, 2× LiTaqTM plus PCR mix, 2× LiTaqTM Eco PCR mix
Competitors: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F
PCR reaction setup for amplification:
PCR cycling for amplification:
From the above results, it can be concluded that for PCR amplification of different length and kind of templates, all four PCR mixes from our company performed superbly. For general PCR, our products have the broadest applicability, best success rate, highest specificity and maximal yield. In the most difficult situations, when all other PCR mixes failed, at least one of our products still worked.