Virus Packaging Services

Virus Packaging Services

Adenovirus Packaging Service

1. Unsurpassable transduction efficiency (nearly 100% vs. AAV 30~40% and Lentivirus ~30%) in most mammalian cells.
2. No integration into host genome.
3. Compatible with toxic gene of interest (GOI).
4. Induce high-level transient protein expression.
5. Accommodate inserts of up to 7.5 kb.

1. Clone your gene of interest (GOI) into a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2. Obtain initial adenoviral stock.
3. Adenovirus amplification from ~1400 cm2 AdMAX™ 293 cells.
4. Recombinant adenovirus PFU titration.

Turnaround time: 3~4 weeks

Required material: A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).

rAAV Packaging Service

1. Nonpathogenic with least immune response.
2. Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
3. Persistent transgene expression.
4. Multiple serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ).

1. rAAV packaging in 10x150 mm plate.
2. rAAV purification via advanced 2xCsCl ultra-centrifugation.
3. Desalting, filter sterilization and AAV titration via qPCR.

Turnaround time: 1~2 weeks

Required material: AAV cis plasmid carrying your gene of interest (GOI), >100 µg.

Lentivirus Packaging Service

1. Derived from 3rd generation packaging system with a shRNA included for maximum yield.
2. An inhibitory trans element upstream of 5'-LTR allows packaging toxic transgenes.
3. LentiMAX™ packaging cell line is optimized for high pseudoviral titers.
4. Compatible with both 2nd and 3rd generation lentivectors.

1. Lentivirus packaging via LentiMAX™ packaging system.
2. Lentivirus purification via PEG precipitation followed by ultra-centrifugation.
3. Recombinant lentivirus titration via p24 ELISA.

Turnaround time: 1~2 weeks

Required material: Lentivector (>50 µg) carrying your gene of interests (GOI) in transfection grade.

Online Quote

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