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LiQuant™ Probe qPCR Master Mix is a special reagent designed for probe based qPCR. Utilizing hot-start LiQuant™ DNA polymerase, alone with optimal buffer specially optimized for qPCR, it is capable of significant restraining non-specific amplification as well as enhancing the amplification efficiency, thus is applicable for high-sensitivity qPCR reactions. It is a 2× premixed reagent which is convenient to handle. When using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.
Key Features
• The mix, prepared at 2× reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability.
• ROX reference dyes are provided for different qPCR instruments.
Components
1. LiQuant™ Probe qPCR Master Mix: 5 ml
2. 50× ROX Reference Dye 1: 200 μl
3. 50× ROX Reference Dye 2: 200 μl
Storage
Store at -20°C and protect from light.
Case Study
When amplifying human GAPDH with serial diluted cDNA from HeLa cells with LiQuant™ Probe qPCR Master Mix or Brand Q product and 100nM TaqMan probe, LiQuant™ Probe qPCR Master Mix shows higher amplification efficiency, lower detection limit,and better linear relationship.Tested on ABl StepOne Plus. (Right)
