LiQuant™ Probe qPCR Master Mix (100 rxns)

LiQuant™ Probe qPCR Master Mix is a TaqMan probe-based reagent specifically designed for quantitative PCR (qPCR). Its core component, LiTaq DNA Polymerase, is a chemically modified hot-start DNA polymerase. Combined with an optimized qPCR buffer system, this master mix effectively suppresses non-specific amplification while significantly enhancing amplification efficiency, making it ideal for high-sensitivity qPCR applications. As a 2× concentrated master mix, the probe qPCR mix enables the generation of excellent standard curves across a broad quantitative range, ensuring accurate quantification and detection of target genes with high repeatability and reliability. 
 

Key Features

• Superior amplification sensitivity: The hot-start LiTaq DNA Polymerase delivers exceptional amplification performance and sensitivity for detecting low-abundance targets.

• Excellent linear relationship: Consistent amplification efficiency of ~99% ensures reliable standard curve generation across wide dynamic ranges.

• Robust Anti-Contamination System: The integrated dUTP/UDG system effectively neutralizes aerosol-borne amplicon contamination, minimizing result deviations and enhancing data integrity.
 

Components

1. LiQuant™ Probe qPCR Master Mix: 1ml
2. 50× ROX Reference Dye 1: 100 µl
3. 50× ROX Reference Dye 2: 100 µl

Storage

Store at -20°C and protect from light.

Case Study

When amplifying human GAPDH with serial diluted cDNA from HeLa cells with LiQuant™ Probe qPCR Master Mix or Brand Q product and 100nM TaqMan probe, LiQuant™ Probe qPCR Master Mix shows higher amplification efficiency, lower detection limit, and better linear relation ship. Tested on ABl StepOne Plus. (Right)