|Key Feature:|| |
• cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
• 5 minutes cDNA synthesis.
• Passive reference dyes are provided for different qPCR instruments.
LiQuantTM One-Step Green RT-qPCR kit combines the first-strand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. LiScriptTM One-Step Green qRT-PCR kit contains all the necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene specific primers.
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• Viral RNA and trace RNA detection
1. RNase free ddH2O: 1.25 ml×2
2. 2× LiScriptTM One Step Green Mix: 1.25 ml×2
3. One Step Green Enzyme Mix: 250 μl
4. 50× ROX Reference Dye 1: 100 μl
4. 50× ROX Reference Dye 2: 100 μl