Labeled Nucleotides

Labeled Nucleotides

LifeSct offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable LiFluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.

• Bright signals with low background
• Wide variety of fluorophores
• Great for many applications, like Fluorescence in situ Hybridization (FISH), TUNEL, and more

Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with LiFluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with LiFluor 488-X-dUTP (green) and LiFluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with LiFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).

Product Name
Cat. #
5-Aminoallyl-dUTPM0070100 μl$149
5-Aminoallyl-UTPM0071100 μl$149
Biotin-11-dUTPM007250 μl$199
Biotin-11-UTPM007350 μl
Biotin-14-dCTPM007450 nmol
5-FAM-dUTPM007525 μl$169
5-FAM-UTPM007625 μl
5-FAM-X-UTPM007725 μl$199
5-FAM-X-UTPM007825 μl
LiFluor™ 488-X-dUTPM007925 μl$299
LiFluor™ 488-X-UTPM008025 μl
LiFluor™ 555-X-dUTPM008125 μl$299
LiFluor™ 555-X-UTPM008225 μl
LiFluor™ 568-X-dUTPM008325 μl$299
LiFluor™ 568-X-UTPM008425 μl
LiFluor™ 594-X-dUTPM008525 μl$299
LiFluor™ 594-X-UTPM008625 μl
LiFluor™ 647-X-dUTPM008725 μl$299
LiFluor™ 647-X-UTPM008825 μl

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